| Registro completo |
| Provedor de dados: |
OAK
|
| País: |
Japan
|
| Título: |
Application of crude and recombinant ELISAs and immunochromatographic test for serodiagnosis of animal trypanosomosis in the Umkhanyakude district of KwaZulu-Natal province, South Africa
|
| Autores: |
NGUYEN, Thu-Thuy
MOTSIRI, Mono Sophie
TAIOE, Moeti Oriel
MTSHALI, Moses Sibusiso
GOTO, Yasuyuki
KAWAZU, Shin-Ichiro
Molifi THEKISOE, Oriel Matlhahane
INOUE, Noboru
河津, 信一郎
井上, 昇
|
| Data: |
2015
|
| Ano: |
2015
|
| Palavras-chave: |
Animal African trypanosomosis
ELISA
Immunochromatographic test
Serodiagnosis
South Africa
|
| Resumo: |
A total of 231 serum samples were collected from sheep (n=9), goats (n=99) and cattle (n=123) in northeastern KwaZulu-Natal, South Africa. Trypanosome infection was detected using Trypanosoma brucei brucei crude antigen (TbbCA) and T. congolense crude antigen (TcoCA) ELISA assays. Recombinant antigen (T. evansi GM6 which consisted of 4 repeat domains, TeGM6-4r) ELISA and immunochromatographic test (ICT) were also used. Crude antigen ELISA, TeGM6-4r-ELISA and ICT detected 27.3%, 29% and 19.9% of trypanosome seropositive samples, respectively. Trypanosome infection prevalence in cattle and goats was 35.8–46.3% and 0–9.1%, respectively. Out of 9 sheep serum samples, 2–4 sera (22.2–44.4%) were positive. The detection performance of crude and recombinant antigen ELISAs was relatively similar (K=0.6–0.7); both are recommended for reference diagnosis and large scale epidemiological surveys. There is potential application for ICT in on-site diagnosis, but its sensitivity should be improved.
https://www.jstage.jst.go.jp/article/jvms/77/2/77_14-0330/_article/-char/ja/
|
| Idioma: |
Inglês
|
| Identificador: |
http://ir.obihiro.ac.jp/dspace/handle/10322/4034
|
| Editor: |
日本獣医学会
|
| Direitos: |
日本獣医学会
|
|
